Boston Molecules

Xactive Recombinant Cas-9 Protein

Be the first to review this product

Availability: In stock

$195.00
OR

Quick Overview

Cas9 Nuclease, S. pyogenes

Xactive Recombinant Cas-9 Protein

Details

Background

The CRISPR/Cas9 system is a powerful tool for gene editing which transform research at an astonishing rate. To achieve maximum editing efficiency across a broad spectrum of cell types, including iPSCs and primary cells, we’ve refined and optimized rate-limiting components of CRISPR/Cas9 system. Our XactiveTM Recombinant Cas9 protein (Electroporation-Ready) was purified from E. coli. utilized folding optimization strategies which maximized Cas9 enzyme activity by achieving functional knockout in up to 98% of primary hematopoietic stem cells (HSCs) (Figure 1).

High editing efficiency without further selection after transfection is especially important when working with difficult-to-edit genomic loci and cells more resistant to protein or DNA delivery such as primary cells, immune cells, and stem cells. we were able to demonstrate over 2 fold higher editing efficiency of easy-to-edit chromosome regions, and more than 2 to 5 fold higher editing efficiency of difficult-to-edit genomic loci than competitors in the market for functional knockout of genes in donor-derived primary cell populations (Figure 1&2).

  Figure 1                  

Figure 1. High-efficiency functional knockout in primary hematopoietic stem cells (HSCs). HSCs cells were isolated from PBMCs (from a healthy donor), and then transfected with XactiveTM Cas9 Protein and sgRNAs targeting hemoglobin subunit beta (HBB) gene regions using electroporation. *represents competitor Cas9.

Figure 2

Figure 2. High-efficiency functional knockout in primary hematopoietic stem cells (HSCs). HSCs cells were isolated from PBMCs (from a healthy donor), and then transfected with XactiveTM Cas9 Protein and sgRNAs targeting hemoglobin subunit beta (HBB) enhancer regions using electroporation. *represents competitor Cas9.


Additional Information

Sample Type Recombinant Protein
Catalog Name P1011
CROSS REACTS WITH No
PRODUCT OVERVIEW No
INTENDED USE Research Use Only
STORAGE INSTRUCTIONS Store at –70°C. Avoid repeated freeze/thaw cycles.
COMPONENTS No
SAFETY NOTES No

Product Tags

Use spaces to separate tags. Use single quotes (') for phrases.